Part:BBa_K950001:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQf621c8210d4d03b1-partinfo-00000000-QINU UNIQf621c8210d4d03b1-partinfo-00000001-QINU

••• HUST-China

Pgal1+rox1+cyc1_terminator Figure 1: The whole circuit Modeling Rate of basic transcription for Panb1: Since promoter Anb1 has basic transcription rate, Rox1, Si-tag and Mcfp-3 would be expressed even in darkness. Hence, to figure out how it takes effect on our model, we run the simulation with different rate of Panb1 basic transcription (0.1*Vmax to 0.2*Vmax with step of 0.005*Vmax). Results: Our DDEs model is sensitive to the amount of basic transcription of Panb1. If the rate of pAnb1 basic transcription increases from 0.100*Vmax to 0.200*Vmax, the amount of Rox1 would become 125 percent. Therefore, with less basic transcription of Panb1, less Rox1 would be expressed and the repressed expression of Si-tag and Mcfp-3 would recover more quickly. We can conclude that our circuit could be improved by reducing the basic transcription of pAnb1. Sequence and Features Assembly Compatibility: 10 COMPATIBLE WITH RFC[10] 12 COMPATIBLE WITH RFC[12] 21 INCOMPATIBLE WITH RFC[21] Illegal BglII site found at 1012 23 COMPATIBLE WITH RFC[23] 25 INCOMPATIBLE WITH RFC[25] Illegal AgeI site found at 84 Illegal AgeI site found at 1224 1000 INCOMPATIBLE WITH RFC[1000] Illegal BsaI site found at 1005 Illegal BsaI.rc site found at 1055

;